Cusabio mouse bmp6 elisa kit

Hepatic <t>BMP6</t> expression is increased in an animal model of APAP-induced ALF. A Representative 4X and 10X images of H&E and percentage of necrotic area quantification (%). B ALT and AST activity determination in serum samples. C and D Hmox1 and Bmp6 mRNA levels determined by RT-qPCR and normalized to 36b4 gene expression. Data are expressed as fold of change relative to control condition (Control). E Correlation of Bmp6 mRNA expression with Hmox1 mRNA expression. F Representative 10X and 20X images of BMP6 immunostaining from liver sections and its quantification. Data are expressed as arbitrary units (a.u.). G Correlation of BMP6 hepatic expression with percentage of necrotic area. Experimental conditions: Mice i.p. injected with vehicle (saline) or APAP (300 mg/kg) and sacrificed 6 or 24 h after APAP administration ( n = 12 animals per group). ** p < 0.01, *** p < 0.005 and **** p < 0.0001, APAP 6 h or 24 h vs. Control

Mouse Bmp6 Elisa Kit, supplied by Cusabio, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/mouse+bmp6+elisa+kit/Mouse+Bone+morphogenetic+protein+6%2CBMP-6+ELISA+Kit/pmc11821676-65-9-27
Average 92 stars, based on 1 article reviews

mouse bmp6 elisa kit - by Bioz Stars, 2026-11

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mouse bmp-6 elisa kit picokine (Boster Bio)

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Mouse BMP-6 ELISA Kit PicoKine® (96 Tests). Quantitate Mouse Bmp6 in bone tissue, cell culture supernatants and serum. Sensitivity: 10pg/ml. The brand Picokine indicates this is a premium quality ELISA kit. Each Picokine kit delivers

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mouse bmp-6 elisa kit (colorimetric) (Novus Biologicals)

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Mouse BMP-6 ELISA Kit (Colorimetric)

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mouse bmp 6 bone morphogenetic protein 6 elisa kit (Elabscience)

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elisa kit bmp 6 mouse (G Biosciences)

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An ELISA kit for the detection of BMP 6 Mouse This uses Sandwich ELISA Double Antibody and has a sensitivity of 46 875pg ml

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mouse bmp6 elisa kit (OriGene)

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For Quantitative Detection of mouse BMP 6 in bone tissue cell culture supernates and serum

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mouse bone morphogenetic protein 6 (bmp6) elisa kit (Bioss)

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Hepatic BMP6 expression is increased in an animal model of APAP-induced ALF. A Representative 4X and 10X images of H&E and percentage of necrotic area quantification (%). B ALT and AST activity determination in serum samples. C and D Hmox1 and Bmp6 mRNA levels determined by RT-qPCR and normalized to 36b4 gene expression. Data are expressed as fold of change relative to control condition (Control). E Correlation of Bmp6 mRNA expression with Hmox1 mRNA expression. F Representative 10X and 20X images of BMP6 immunostaining from liver sections and its quantification. Data are expressed as arbitrary units (a.u.). G Correlation of BMP6 hepatic expression with percentage of necrotic area. Experimental conditions: Mice i.p. injected with vehicle (saline) or APAP (300 mg/kg) and sacrificed 6 or 24 h after APAP administration ( n = 12 animals per group). ** p < 0.01, *** p < 0.005 and **** p < 0.0001, APAP 6 h or 24 h vs. Control

Journal: Archives of Toxicology

Article Title: BMP6 participates in the molecular mechanisms involved in APAP hepatotoxicity

doi: 10.1007/s00204-024-03954-5

Figure Lengend Snippet: Hepatic BMP6 expression is increased in an animal model of APAP-induced ALF. A Representative 4X and 10X images of H&E and percentage of necrotic area quantification (%). B ALT and AST activity determination in serum samples. C and D Hmox1 and Bmp6 mRNA levels determined by RT-qPCR and normalized to 36b4 gene expression. Data are expressed as fold of change relative to control condition (Control). E Correlation of Bmp6 mRNA expression with Hmox1 mRNA expression. F Representative 10X and 20X images of BMP6 immunostaining from liver sections and its quantification. Data are expressed as arbitrary units (a.u.). G Correlation of BMP6 hepatic expression with percentage of necrotic area. Experimental conditions: Mice i.p. injected with vehicle (saline) or APAP (300 mg/kg) and sacrificed 6 or 24 h after APAP administration ( n = 12 animals per group). ** p < 0.01, *** p < 0.005 and **** p < 0.0001, APAP 6 h or 24 h vs. Control

Article Snippet: Serum BMP6 concentration was quantified by ELISA using the mouse BMP6 ELISA kit (CSB-E09279m) for mouse samples and the human BMP6 ELISA kit (CSB-E09277h) for human samples (Cusabio Technology LLC, Hubei, China), following the manufacturer ́s instructions.

Techniques: Expressing, Animal Model, Activity Assay, Quantitative RT-PCR, Gene Expression, Control, Immunostaining, Injection, Saline

Circulating BMP6 is increased in mice after APAP overdose and in patients with APAP intoxication. Experimental conditions: Mice i.p. injected with vehicle (saline) or APAP (300 mg/kg) and sacrificed 6 or 24 h after APAP administration ( n = 12 animals per group). A Serum levels of BMP6 determined by ELISA. Data are expressed as pg/ml. B and C Correlation of serum BMP6 levels with hepatic expression of BMP6, circulating ALT and AST, respectively. Study population: 18 patients with APAP overdose, 9 with DILI (ALT > 100 U/L) and 9 without DILI. D Serum levels of BMP6 determined by ELISA. Data are expressed as pg/ml. E Correlation of serum BMP6 levels with circulating ALT levels. ** p < 0.01 and *** p < 0.005, APAP 6 h or 24 h vs. Control

Journal: Archives of Toxicology

Article Title: BMP6 participates in the molecular mechanisms involved in APAP hepatotoxicity

doi: 10.1007/s00204-024-03954-5

Figure Lengend Snippet: Circulating BMP6 is increased in mice after APAP overdose and in patients with APAP intoxication. Experimental conditions: Mice i.p. injected with vehicle (saline) or APAP (300 mg/kg) and sacrificed 6 or 24 h after APAP administration ( n = 12 animals per group). A Serum levels of BMP6 determined by ELISA. Data are expressed as pg/ml. B and C Correlation of serum BMP6 levels with hepatic expression of BMP6, circulating ALT and AST, respectively. Study population: 18 patients with APAP overdose, 9 with DILI (ALT > 100 U/L) and 9 without DILI. D Serum levels of BMP6 determined by ELISA. Data are expressed as pg/ml. E Correlation of serum BMP6 levels with circulating ALT levels. ** p < 0.01 and *** p < 0.005, APAP 6 h or 24 h vs. Control

Techniques: Injection, Saline, Enzyme-linked Immunosorbent Assay, Expressing, Control

Immortalized mouse hepatocytes showed elevated expression and release of BMP6 after APAP treatment. A Cell viability determined by crystal violet staining. Data are expressed as percentage relative to control condition (100%). B Cytotoxicity determined by lactate dehydrogenase (LDH) release. Data are expressed as percentage relative to the positive control (100%). C Representative 40X images of DAPI staining. D ROS production detected with DHE probe represented as DHE fluorescence F/F 0 (a.u.). E Representative blots of the cell lysates with the indicated antibodies. F Bmp6 mRNA levels determined by RT-qPCR and normalized to 36b4 gene expression. Data are expressed as fold of change relative to control condition. G Representative blot of the cultured media (CM) with BMP6 antibody. Ponceau staining was used as loading control. Experimental conditions: immortalized mouse hepatocytes treated with 1 mM or 5 mM APAP for 2 (D), 6 (E) or 16 (A, B, C, F, G) hours (N > 3 independent experiments). ** p < 0.01, *** p < 0.005 and **** p < 0.0001, 1 or 5 mM APAP vs. non-treated cells

Journal: Archives of Toxicology

Article Title: BMP6 participates in the molecular mechanisms involved in APAP hepatotoxicity

doi: 10.1007/s00204-024-03954-5

Figure Lengend Snippet: Immortalized mouse hepatocytes showed elevated expression and release of BMP6 after APAP treatment. A Cell viability determined by crystal violet staining. Data are expressed as percentage relative to control condition (100%). B Cytotoxicity determined by lactate dehydrogenase (LDH) release. Data are expressed as percentage relative to the positive control (100%). C Representative 40X images of DAPI staining. D ROS production detected with DHE probe represented as DHE fluorescence F/F 0 (a.u.). E Representative blots of the cell lysates with the indicated antibodies. F Bmp6 mRNA levels determined by RT-qPCR and normalized to 36b4 gene expression. Data are expressed as fold of change relative to control condition. G Representative blot of the cultured media (CM) with BMP6 antibody. Ponceau staining was used as loading control. Experimental conditions: immortalized mouse hepatocytes treated with 1 mM or 5 mM APAP for 2 (D), 6 (E) or 16 (A, B, C, F, G) hours (N > 3 independent experiments). ** p < 0.01, *** p < 0.005 and **** p < 0.0001, 1 or 5 mM APAP vs. non-treated cells

Techniques: Expressing, Staining, Control, Positive Control, Fluorescence, Quantitative RT-PCR, Gene Expression, Cell Culture

Huh7 hepatocytes release BMP6 after APAP exposure. A Cell viability determined by crystal violet staining. Data are expressed as percentage relative to control condition (100%). B Cytotoxicity determined by lactate dehydrogenase (LDH) release. Data are expressed as percentage relative to the positive control (100%). C Representative 40X images of DAPI staining. D ROS production detected with DHE probe represented as DHE fluorescence F/F 0 (a.u.). E Representative blots of the cell lysates with the indicated antibodies. F BMP6 mRNA levels determined by RT-qPCR and normalized to 36B4 gene expression. Data are expressed as fold of change relative to control condition. G Representative blot of the cultured media (CM) with BMP6 antibody. Ponceau staining was used as loading control. Experimental conditions: Huh7 treated with 10 mM and 20 mM APAP for 2 (D), 6 (E) or 16 (A, B, C, F, G) hours ( N > 3 independent experiments). **** p < 0.0001, 10 or 20 mM APAP vs. non-treated cells

Journal: Archives of Toxicology

Article Title: BMP6 participates in the molecular mechanisms involved in APAP hepatotoxicity

doi: 10.1007/s00204-024-03954-5

Figure Lengend Snippet: Huh7 hepatocytes release BMP6 after APAP exposure. A Cell viability determined by crystal violet staining. Data are expressed as percentage relative to control condition (100%). B Cytotoxicity determined by lactate dehydrogenase (LDH) release. Data are expressed as percentage relative to the positive control (100%). C Representative 40X images of DAPI staining. D ROS production detected with DHE probe represented as DHE fluorescence F/F 0 (a.u.). E Representative blots of the cell lysates with the indicated antibodies. F BMP6 mRNA levels determined by RT-qPCR and normalized to 36B4 gene expression. Data are expressed as fold of change relative to control condition. G Representative blot of the cultured media (CM) with BMP6 antibody. Ponceau staining was used as loading control. Experimental conditions: Huh7 treated with 10 mM and 20 mM APAP for 2 (D), 6 (E) or 16 (A, B, C, F, G) hours ( N > 3 independent experiments). **** p < 0.0001, 10 or 20 mM APAP vs. non-treated cells

Techniques: Staining, Control, Positive Control, Fluorescence, Quantitative RT-PCR, Gene Expression, Cell Culture

Silencing of BMP6 in Huh7 cells does not affect APAP-induced cell death. Experimental conditions (A–E): siBMP6 and control (siC) Huh7 cells treated with 20 mM APAP for 16 h ( N > 3 independent experiments). A BMP6 mRNA levels determined by RT-qPCR and normalized to 36B4 gene expression. Data are expressed as fold of change relative to control condition (siC-C). B Representative blots of the cell lysates with the indicated antibodies and the corresponding quantification. Data are expressed as fold of change relative to control condition (siC). C Cell viability determined by crystal violet staining. Data are expressed as percentage relative to control condition (C, 100%). D Cytotoxicity determined by lactate dehydrogenase (LDH) release. Data are expressed as percentage relative to the positive control (100%). E Representative blots of the cell lysates with the indicated antibodies. F Experimental conditions (F–I): siBMP6 and control (siC) Huh7 cells treated with 20 mM APAP for 16 h. Huh7 cells incubated with siBMP6- or siC-CM for 24 h ( N > 3 independent experiments). G Representative blot of the cultured media (CM) with BMP6 antibody. Ponceau staining was used as loading control. H . Cell viability determined by crystal violet staining. Data are expressed as percentage relative to control condition (C, 100%). I Cytotoxicity determined by lactate dehydrogenase (LDH) release. Data are expressed as percentage relative to the positive control (100%). * p < 0.05, *** p < 0.005 and **** p < 0.0001, APAP vs. C; # p < 0.05 and ## p < 0.01, siBMP6 vs. siC

Journal: Archives of Toxicology

Article Title: BMP6 participates in the molecular mechanisms involved in APAP hepatotoxicity

doi: 10.1007/s00204-024-03954-5

Figure Lengend Snippet: Silencing of BMP6 in Huh7 cells does not affect APAP-induced cell death. Experimental conditions (A–E): siBMP6 and control (siC) Huh7 cells treated with 20 mM APAP for 16 h ( N > 3 independent experiments). A BMP6 mRNA levels determined by RT-qPCR and normalized to 36B4 gene expression. Data are expressed as fold of change relative to control condition (siC-C). B Representative blots of the cell lysates with the indicated antibodies and the corresponding quantification. Data are expressed as fold of change relative to control condition (siC). C Cell viability determined by crystal violet staining. Data are expressed as percentage relative to control condition (C, 100%). D Cytotoxicity determined by lactate dehydrogenase (LDH) release. Data are expressed as percentage relative to the positive control (100%). E Representative blots of the cell lysates with the indicated antibodies. F Experimental conditions (F–I): siBMP6 and control (siC) Huh7 cells treated with 20 mM APAP for 16 h. Huh7 cells incubated with siBMP6- or siC-CM for 24 h ( N > 3 independent experiments). G Representative blot of the cultured media (CM) with BMP6 antibody. Ponceau staining was used as loading control. H . Cell viability determined by crystal violet staining. Data are expressed as percentage relative to control condition (C, 100%). I Cytotoxicity determined by lactate dehydrogenase (LDH) release. Data are expressed as percentage relative to the positive control (100%). * p < 0.05, *** p < 0.005 and **** p < 0.0001, APAP vs. C; # p < 0.05 and ## p < 0.01, siBMP6 vs. siC

Techniques: Control, Quantitative RT-PCR, Gene Expression, Staining, Positive Control, Incubation, Cell Culture

BMP6 promotes THP1 polarization toward an anti-inflammatory phenotype (M2). A Experimental conditions: siBMP6 and control (siC) Huh7 hepatocytes treated with 20 mM APAP for 16 h. THP1 cells incubated with siBMP6- or siC-CM for 24 h (N > 3 independent experiments). B, C, D and E mRNA levels of IL4, IL13, ARG1, MRC1, IL1B, IL6 and TNFA determined by RT-qPCR and normalized to 36B4 gene expression. Data are expressed as fold of change relative to the control condition (C or -). Experimental conditions: ( D and E ) THP1 treated with BMP6 (1, 10 and 100 ng/ml) for 24 h ( N > 3 independent experiments performed by duplicate). * p < 0.05, ** p < 0.01, *** p < 0.005 and **** p < 0.0001, APAP vs. C, or BMP6 vs. -; # p < 0.05 siBMP6 CM vs. siC CM

Journal: Archives of Toxicology

Article Title: BMP6 participates in the molecular mechanisms involved in APAP hepatotoxicity

doi: 10.1007/s00204-024-03954-5

Figure Lengend Snippet: BMP6 promotes THP1 polarization toward an anti-inflammatory phenotype (M2). A Experimental conditions: siBMP6 and control (siC) Huh7 hepatocytes treated with 20 mM APAP for 16 h. THP1 cells incubated with siBMP6- or siC-CM for 24 h (N > 3 independent experiments). B, C, D and E mRNA levels of IL4, IL13, ARG1, MRC1, IL1B, IL6 and TNFA determined by RT-qPCR and normalized to 36B4 gene expression. Data are expressed as fold of change relative to the control condition (C or -). Experimental conditions: ( D and E ) THP1 treated with BMP6 (1, 10 and 100 ng/ml) for 24 h ( N > 3 independent experiments performed by duplicate). * p < 0.05, ** p < 0.01, *** p < 0.005 and **** p < 0.0001, APAP vs. C, or BMP6 vs. -; # p < 0.05 siBMP6 CM vs. siC CM

Techniques: Control, Incubation, Quantitative RT-PCR, Gene Expression

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